Journal:

Article Title: Remodeling of the Vessel Wall after Copper-Induced Injury Is Highly Attenuated in Mice with a Total Deficiency of Plasminogen Activator Inhibitor-1

doi:

Figure Lengend Snippet: Transmission electron microscopy of carotid arteries of WT and PAI-1−/− mice 3 days after cuff implantation. A: A neutrophil with vesicular transport activity (arrow) is seen attached to the luminal endothelium in WT carotid artery close to a cellular junction (asterisk) (original magnification, ×11,500). B: A polymorphonuclear leukocyte (arrow) is present in an open subendothelial space in WT carotid artery (original magnification, ×6,200). A and B suggest that a diapedesis process is occurring. C: Altered cellular morphology of an endothelial cell (E) is evident in PAI-1−/− carotid artery, the result of copper-induced injury (original magnification, ×6,800). D: Activated macrophages with visible phagolysosomes (P) are evident in the medial compartment of a PAI-1−/− carotid artery (original magnification, ×2,700).

Article Snippet: PAI-1 expression in the vessel wall was evaluated by immunohistochemistry using a rabbit anti-murine PAI-1 polyclonal antibody (Molecular Innovations, Inc., Royal Oak, MI).

Techniques: Transmission Assay, Electron Microscopy, Activity Assay

Journal:

Article Title: Remodeling of the Vessel Wall after Copper-Induced Injury Is Highly Attenuated in Mice with a Total Deficiency of Plasminogen Activator Inhibitor-1

doi:

Figure Lengend Snippet: Histological analysis of carotid arteries from WT and PAI-1−/− mice 7 days after implantation of the copper cuff. A: H&E stain of WT carotid artery demonstrates a reactive adventitial (asterisk) and medial (arrow) compartments with the presence of a small neointima (arrowhead). Original magnification, ×200. Similar H&E staining of a carotid artery from a PAI-1−/− mouse (B) shows a quiescent state of these vascular compartments. Original magnification, ×200. C: Anti-α-actin immunostain of a WT carotid artery demonstrates enhanced smooth muscle cells in the medial compartment (arrow) (original magnification, ×200), whereas a PAI-1−/− carotid artery (D) indicates no apparent change in the smooth muscle cell population in the medial compartment. Original magnification, ×200. E: CD45 immunostain of WT carotid artery demonstrates a leukocyte-enriched adventitia (arrow). Original magnification, ×200. F: CD45 immunostain of WT carotid artery demonstrates leukocytes within the neointima (arrow). Original magnification, ×400. G: Anti-fibrin(ogen) immunostain of WT carotid artery shows fibrin-rich medial (arrow) and adventitial (asterisk) compartments (original magnification, ×200), whereas similar immunostaining of a PAI-1−/− carotid artery (H) demonstrates no apparent fibrin deposition in the vessel wall. Original magnification, ×200.

Article Snippet: PAI-1 expression in the vessel wall was evaluated by immunohistochemistry using a rabbit anti-murine PAI-1 polyclonal antibody (Molecular Innovations, Inc., Royal Oak, MI).

Techniques: Staining, Immunostaining

Journal:

Article Title: Remodeling of the Vessel Wall after Copper-Induced Injury Is Highly Attenuated in Mice with a Total Deficiency of Plasminogen Activator Inhibitor-1

doi:

Figure Lengend Snippet: Vessel counts per mm 2 area of the adventitial compartment of arteries from WT and PAI-1−/− mice 7 and 21 days after perivascular placement of the cuff. WT versus PAI-1−/− at day 21, P = 0.0057.

Article Snippet: PAI-1 expression in the vessel wall was evaluated by immunohistochemistry using a rabbit anti-murine PAI-1 polyclonal antibody (Molecular Innovations, Inc., Royal Oak, MI).

Techniques:

Journal: Virology Journal

Article Title: Comparison of enzyme-linked immunosorbent assay systems using rift valley fever virus nucleocapsid protein and inactivated virus as antigens

doi: 10.1186/s12985-018-1071-y

Figure Lengend Snippet: Western-blot analysis of purified RVFV-N protein. Pre-stained protein marker and purified recombinant RVFV-N protein were separated by SDS-PAGE and transferred to a PVDF membrane. Each membrane was incubated with diluted rabbit RVF hyper-immune sera or mouse anti-Histidine serum followed by horseradish peroxidase(HRP)-conjugated anti-rabbit IgG or anti-mouse IgG (1:1000 dilution) and detected by DAB staining. a Reactivity of recombinant protein to rabbit RVF hyper-immune sera. b Reactivity of recombinant protein to mouse anti-Histidine antibody. Lane 1: protein marker (Precision plus protein standards, Bio-Rad); Lane 2: purified RVFV-N protein

Article Snippet: Briefly, for each sample to be tested, 4 wells were subjected to the following reaction steps: coating with anti-RVFV hyper-immune mouse ascetic fluid (HMAF) diluted at 1:8000; then, addition of 1:18 dilution of inactivated RVFV culture fluid (positive antigen) to two of these wells and to the other two wells with 1:18 dilution of mock culture fluid (negative antigen); followed by the addition of test serum samples (diluted 1:100) to all the 4 wells; and then, detection of bound IgG by 1:8000 diluted peroxidase-conjugated mouse anti-human IgG (Fc specific) (Accurate Chemical & Scientific Corp, Westbury, NY) after which the ABTS substrate was added to produce a color reaction for OD detection.

Techniques: Western Blot, Purification, Staining, Marker, Recombinant, SDS Page, Incubation